Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Genome position and gene amplification. | Research Open Access Genome position and gene amplification Pavla Gajduskova Antoine M Snijders Serena Kwek Ritu Roydasgupta Jane Fridlyand Taku Tokuyasu Daniel PinkeF and Donna G Albertson Addresses Cancer Research Institute University of California San Francisco San Francisco CA 94143-0808 USA. Comprehensive Cancer Center University of California San Francisco San Francisco CA 94143-0808 USA. Department of Epidemiology and Biostatistics University of California San Francisco San Francisco CA 94143-0808 USA. Department of Laboratory Medicine University of California San Francisco San Francisco CA 94143-0808 USA. Institute of Biophysics Academy of Sciences of the Czech Republic Královopolská Brno 612 65 Czech Republic. Correspondence Donna G Albertson. Email albertson@ Published 21 June 2007 Genome Biology 2007 8 RI20 doi gb-2007-8-6-rl 20 The electronic version of this article is the complete one and can be found online at http 2007 8 6 R120 Received 6 November 2006 Revised 15 May 2007 Accepted 2l June 2007 2007 Gajduskova et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Amplifications regions of focal high-level copy number change lead to overexpression of oncogenes or drug resistance genes in tumors. Their presence is often associated with poor prognosis however the use of amplification as a mechanism for overexpression of a particular gene in tumors varies. To investigate the influence of genome position on propensity to amplify we integrated a mutant form of the gene encoding dihydrofolate reductase into different positions in the human genome challenged cells with methotrexate and then studied the genomic alterations arising in drug resistant cells. .
