Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns. | Open Access Research Violating the splicing rules TG dinucleotides function as alternative 3 splice sites in U2-dependent introns Karol Szafranski Stefanie Schindler Stefan Taudien Michael Hiller Klaus Huse Niels Jahn Stefan Schreiber Rolf Backofen and Matthias Platzer Addresses Genome Analysis Leibniz Institute for Age Research - Fritz Lipmann Institute Beutenbergstr. 07745 Jena Germany. Institute of Computer Science Bioinformatics Group Albert-Ludwigs-University Freiburg Georges-Koehler-Allee 79110 Freiburg Germany. Institute of Clinical Molecular Biology Christian Albrechts University Kiel Schittenhelmstr. 24105 Kiel Germany. n These authors contributed equally to this work. Correspondence Karol Szafranski. Email szafrans@ Published I August 2007 Genome Biology 2007 8 RI54 doi gb-2007-8-8-rI 54 The electronic version of this article is the complete one and can be found online at http 2007 8 8 RI54 Received 8 March 2007 Revised I4 June 2007 Accepted I August 2007 2007 Szafranski et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cite. Abstract Background Despite some degeneracy of sequence signals that govern splicing of eukaryotic pre-mRNAs it is an accepted rule that U2-dependent introns exhibit the 3 terminal dinucleotide AG. Intrigued by anecdotal evidence for functional non-AG 3 splice sites we carried out a human genome-wide screen. Results We identified TG dinucleotides functioning as alternative 3 splice sites in 36 human genes. The TG-derived splice variants were experimentally validated with a success rate of 92 . Interestingly ratios of alternative splice variants are tissue-specific for several introns. TG splice sites and their flanking intron sequences are substantially .
