Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans. | Open Access Research A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans Ana C Gomes Isabel Miranda Raquel M Silva Gabriela R Moura Benjamin Thomas Alexandre Akoulitchev and Manuel AS Santos Addresses CESAM Department of Biology University of Aveiro 3810-193 Aveiro Portugal. Central Proteomics Facility Sir William Dunn School of Pathology University of Oxford South Parks Road Oxford OX1 3RE UK. Correspondence Manuel AS Santos. Email msantos@ Published 4 October 2007 Genome Biology 2007 8 R206 doi gb-2007-8- l0-r206 The electronic version of this article is the complete one and can be found online at http 2007 8 10 R206 Received 10 May 2007 Revised 31 July 2007 Accepted 4 October 2007 2007 Gomes et al licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genetic code alterations have been reported in mitochondrial prokaryotic and eukaryotic cytoplasmic translation systems but their evolution and how organisms cope and survive such dramatic genetic events are not understood. Results Here we used an unusual decoding of leucine CUG codons as serine in the main human fungal pathogen Candida albicans to elucidate the global impact of genetic code alterations on the proteome. We show that C. albicans decodes CUG codons ambiguously and tolerates partial reversion of their identity from serine back to leucine on a genome-wide scale. Conclusion Such codon ambiguity expands the proteome of this human pathogen exponentially and is used to generate important phenotypic diversity. This study highlights novel features of C. albicans biology and unanticipated roles for codon ambiguity in the evolution of the genetic code. Background Since the .
