Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes. | Open Access Research GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts an in silico dissection of mixed phenotypes Shi-Jiang Lun Jennifer A HippHt Qiang Feng Jason D Hipp Robert Lanza and Anthony Atala Addresses Advanced Cell Technology Worcester MA 01605 USA. Institute of Regenerative Medicine Wake Forest University School of Medicine Winston-Salem NC 27157 USA. n These authors contributed equally to this work. Correspondence Anthony Atala. Email aatala@ Published 13 November 2007 Genome Biology 2007 8 R240 doi gb-2007-8- 11-r240 The electronic version of this article is the complete one and can be found online at http 2007 8 11 R240 Received 11 June 2007 Revised 10 July 2007 Accepted 1 3 November 2007 2007 Lu et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Microarrays are being used to understand human embryonic stem cell hESC differentiation. Most differentiation protocols use a multi-stage approach that induces commitment along a particular lineage. Therefore each stage represents a more mature and less heterogeneous phenotype. Thus characterizing the heterogeneous progenitor populations upon differentiation are of increasing importance. Here we describe a novel method of data analysis using a recently developed differentiation protocol involving the formation of functional hemangioblasts from hESCs. Blast cells are multipotent and can differentiate into multiple lineages of hematopoeitic cells erythroid granulocyte and macrophage endothelial and smooth muscle cells. Results Large-scale transcriptional analysis was performed at distinct time points of hESC differentiation undifferentiated hESCs embryoid bodies and blast cells
