Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Direct selection and phage display of a Gram-positive secretome. | Method Open Access Direct selection and phage display of a Gram-positive secretome Dragana Jankovic Michael A Collett Mark W Lubbers and Jasna Rakonjac Addresses institute of Molecular Biosciences Massey University Palmerston North New Zealand. Tonterra Research Centre Palmerston North New Zealand. Fonterra Mount Waverley VIC 3149 Australia. Correspondence Jasna Rakonjac. Email Published 13 December 2007 Genome Biology 2007 8 R266 doi gb-2007-8- l2-r266 The electronic version of this article is the complete one and can be found online at http 2007 8 12 R266 Received 29 July 2007 Revised 1 November 2007 Accepted l3 December 2007 2007 Jankovic et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Surface secreted and transmembrane protein-encoding open reading frames collectively the secretome can be identified in bacterial genome sequences using bioinformatics. However functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection identification expression and purification of bacterial secretome proteins. Background The secretome comprises a wide range of proteins that mediate interactions with the environment such as receptors adhesins transporters complex cell surface structures such as pili secreted enzymes toxins and virulence factors. In bacteria that colonize the human organism secreted proteins mediate attachment to the host destruction of the host tissue or interference with the immune response 1-3 . In pathogenic bacteria variation of a surface protein between strains of a species can indicate its role in evading .
