Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity. | Open Access Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity Sara Holland Emma Lodwig Theodora Sideri Tom Reader Ian Clarke Konstantinos Gkargkas David C Hoyle Daniela Delneri Stephen G Oliver and Simon V Avery Addresses School of Biology Institute of Genetics The University of Nottingham University Park Nottingham NG7 2RD UK. North West Institute for Bio-Health Informatics The University of Manchester ISBE School of Medicine Oxford Road Manchester M13 9PT UK. Department of Biochemistry University of Cambridge Sanger Building Tennis Court Road Cambridge CB2 1GA UK. Faculty of Life Sciences The University of Manchester Oxford Road Manchester M13 9PT UK. Correspondence Simon V Avery. Email Published 18 December 2007 Genome Biology 2007 8 R268 doi gb-2007-8- l2-r268 The electronic version of this article is the complete one and can be found online at http 2007 8 12 R268 Received 15 November 2007 Revised 18 December 2007 Accepted 18 December 2007 2008 Holland et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The serious biological consequences of metal toxicity are well documented but the key modes of action of most metals are unknown. To help unravel molecular mechanisms underlying the action of chromium a metal of major toxicological importance we grew over 6 000 heterozygous yeast mutants in competition in the presence of chromium. Microarray-based screens of these heterozygotes are truly genome-wide as they include both essential and nonessential genes. Results The screening data indicated that proteasomal protein degradation activity is crucial for .
