Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Cross-kingdom patterns of alternative splicing and splice recognition. | Open Access Cross-kingdom patterns of alternative splicing and splice recognition Abigail M McGuire Matthew D Pearson Daniel E Neafsey and James E Galagan Address The Broad Institute of MIT and Harvard Cambridge Center Cambridge MA 02142 USA. These authors contributed equally to this work. Correspondence Abigail M McGuire. Email amcguire@ Published 5 March 2008 Genome Biology 2008 9 R50 doi 186 gb-2008-9-3-r50 The electronic version of this article is the complete one and can be found online at http 2008 9 3 R50 Received 15 October 2007 Revised 28 January 2008 Accepted 5 March 2008 2008 Manson McGuire et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Variations in transcript splicing can reveal how eukaryotes recognize intronic splice sites. Retained introns RIs commonly appear when the intron definition ID mechanism of splice site recognition inconsistently identifies intron-exon boundaries and cassette exons CEs are often caused by variable recognition of splice junctions by the exon definition ED mechanism. We have performed a comprehensive survey of alternative splicing across 42 eukaryotes to gain insight into how spliceosomal introns are recognized. Results All eukaryotes we studied exhibit RIs which appear more frequently than previously thought. CEs are also present in all kingdoms and most of the organisms in our analysis. We observe that the ratio of CEs to RIs varies substantially among kingdoms while the ratio of competing 3 acceptor and competing 5 donor sites remains nearly constant. In addition we find the ratio of CEs to RIs in each organism correlates with the length of its introns. In all 14 fungi we examined as well as in most of the 9 protists
