Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Individual mRNA expression profiles reveal the effects of specific microRNAs Amit Arora and David AC Simpson. | Open Access Research Individual mRNA expression profiles reveal the effects of specific microRNAs Amit Arora and David AC Simpson Address Queen s University Belfast Centre for Vision Sciences Institute of Clinical Science Royal Victoria Hospital Belfast BT12 6BA UK. Correspondence David AC Simpson. Email Published 16 May 2008 Received 5 November 2007 Genome Biology 2008 9 R82 doi gb-2008-9-5-r82 Rev eidj 6 M ri 2008 Accepted 16 May 2008 The electronic version of this article is the complete one and can be found online at http 2008 9 5 R82 2008 Arora and Simpson licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background MicroRNAs miRNAs are oligoribonucleotides with an important role in regulation of gene expression at the level of translation. Despite imperfect target complementarity they can also significantly reduce mRNA levels. The validity of miRNA target gene predictions is difficult to assess at the protein level. We sought therefore to determine whether a general lowering of predicted target gene mRNA expression by endogenous miRNAs was detectable within microarray gene expression profiles. Results The target gene sets predicted for each miRNA were mapped onto known gene expression data from a range of tissues. Whether considering mean absolute target gene expression rank sum tests or ranked ratios many miRNAs with significantly reduced target gene expression corresponded to those known to be expressed in the cognate tissue. Expression levels of miRNAs with reduced target mRNA levels were higher than those of miRNAs with no detectable effect on mRNA expression. Analysis of microarray data gathered after artificial perturbation of expression of a specific .
