Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: The costs of breed reconstruction from cryopreserved material in mammalian livestock species | Genet. Sel. Evol. 39 2007 465-479 INRA EDP Sciences 2007 DOI gse 2007015 Available online at Original article The costs of breed reconstruction from cryopreserved material in mammalian livestock species Gustavo GANDlNla Flavia Pizzib Alessandra STELLAc Paul J. BOETTCHERb d a Department VSA Università degli Studi di Milano Milan Italy b Instituto di Biologia e Biotecnologia Agraria CNR Milan Italy c CERSA-Parco Tecnologico Padano Lodi Italy d IAEA FAO Joint Division for Nuclear Techniques in Food and Agriculture Vienna Austria Received 9 August 2006 accepted 9 January 2007 Abstract - The aim of this work was to compare costs in the horse cattle sheep swine and rabbit species for the creation of gene banks for reconstruction of an extinct breed using different strategies embryos-only embryos in combination with semen and semen-only. Three cost measures were used time required for population reconstruction cost for creation of the gene bank number of years-keeping-female to reach reconstruction. Semen costs were estimated across four scenarios the presence or absence of a commercial market for semen purchase of semen donors and semen extracted from the epididymus. The number of cells were doubled to take into account the creation of two storage sites. The strategy embryos-only required the shortest time to reach reconstruction. With the strategy embryos semen time increased with decreasing proportions of embryos. With semen-only reconstruction time varied from 2 to 21 years. A high variation of costs was observed across species and strategies from 360 Euros in the rabbit to 1 092300 in the horse. In all species the embryos-only strategy was about 10 more expensive than using 90 embryos semen. Decreasing the percentage of embryos further diminished costs. The number of years-keeping-female ranged across strategies from 2 in the rabbit to a maximum of 12 878 in the horse. conservation cost genetic conservation breed reconstruction gene bank .
