Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets. | Research Open Access Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets Xiu-Jie WangK José L ReyesHt Nam-Hai Chua and Terry Gaasterland Addresses Laboratory of Computational Genomics The Rockefeller University New York NY 10021 USA. Laboratoiy of Plant Molecular Biology The Rockefeller University New York NY 10021 USA. K These authors contributed equally to this work. Correspondence Terry Gaasterland. E-mail gaasterland@ Published 31 August 2004 Genome Biology 2004 5 R65 The electronic version of this article is the complete one and can be found online at http 2004 5 9 R65 Received 5 April 2004 Revised 22 June 2004 Accepted 2 August 2004 2004 Wang et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A class of eukaryotic non-coding RNAs termed microRNAs miRNAs interact with target mRNAs by sequence complementarity to regulate their expression. The low abundance of some miRNAs and their time- and tissue-specific expression patterns make experimental miRNA identification difficult. We present here a computational method for genome-wide prediction of Arabidopsis thaliana microRNAs and their target mRNAs. This method uses characteristic features of known plant miRNAs as criteria to search for miRNAs conserved between Arabidopsis and Oryza sativa. Extensive sequence complementarity between miRNAs and their target mRNAs is used to predict miRNA-regulated Arabidopsis transcripts. Results Our prediction covered 63 of known Arabidopsis miRNAs and identified 83 new miRNAs. Evidence for the expression of 25 predicted miRNAs came from northern blots their presence in the Arabidopsis Small RNA Project database and massively parallel signature .
