Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-scale approaches for discovering novel nonconventional splicing substrates of the Ire1 nuclease. | Research Open Access Genome-scale approaches for discovering novel nonconventional splicing substrates of the Irel nuclease Maho Niwa Christopher K Patil Joe DeRisi and Peter Walter Addresses Howard Hughes Medical Institute and Department of Biochemistry and Biophysics University of California at San Francisco San Francisco CA 94143-2200 USA. Current address Division of Biology Section of Molecular Biology University of California at San Diego La Jolla CA 92093-0366 USA. Current address Lawrence Berkeley National Laboratory Life Sciences Division 1 Cyclotron Road Berkeley CA 94720 USA. Correspondence Maho Niwa. E-mail niwa@ Published 22 December 2004 Genome Biology 2004 6 R3 The electronic version of this article is the complete one and can be found online at http 2004 6 1 R3 Received 26 August 2004 Revised 17 November 2004 Accepted 25 November 2004 2004 Niwa et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The unfolded protein response UPR allows intracellular feedback regulation that adjusts the protein-folding capacity of the endoplasmic reticulum ER according to need. The signal from the ER lumen is transmitted by the ER-transmembrane kinase Irel which upon activation displays a site-specific endoribonuclease activity. Endonucleolytic cleavage of the intron from the HACI mRNA encoding a UPR-specific transcription factor is the first step in a nonconventional mRNA splicing pathway the released exons are then joined by tRNA ligase. Because only the spliced mRNA is translated splicing is the key regulatory step of the UPR. Results We developed methods to search for additional mRNA substrates of Ire1p in three independent lines of genome-wide analysis. These methods .
