Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Fast and systematic genome-wide discovery of conserved regulatory elements using a non-alignment based approach. | Open Access Methb d Fast and systematic genome-wide discovery of conserved regulatory elements using a non-alignment based approach Olivier Elemento and Saeed Tavazoie Address Lewis-Sigler Institute for Integrative Genomics Princeton University Princeton NJ 08544 USA. Correspondence Saeed Tavazoie E-mail tavazoie@ Published 26 January 2005 Genome Biology 2005 6 The electronic version of this article is the complete one and can be found online at http 2005 6 2 R18 Received 1 September 2004 Revised 29 October 2004 Accepted 3 December 2004 2005 Elemento and Tavazoie licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We describe a powerful new approach for discovering globally conserved regulatory elements between two genomes. The method is fast simple and comprehensive without requiring alignments. Its application to pairs of yeasts worms flies and mammals yields a large number of known and novel putative regulatory elements. Many of these are validated by independent biological observations have spatial and or orientation biases are co-conserved with other elements and show surprising conservation across large phylogenetic distances. Background One of the major challenges facing biology is to reconstruct the entire network of protein-DNA interactions within living cells. A large fraction of protein-DNA interactions corresponds to transcriptional regulators binding DNA in the neighborhood of protein-coding and RNA genes. By interacting with RNA polymerase or recruiting chromatin-modifying machinery transcriptional regulators increase or decrease the transcription rate of these genes. Transcriptional regulators bind specific DNA sequences upstream within or downstream of the .
