Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Genomic analysis of early murine mammary gland development using novel probe-level algorithms. | Method Open Access Genomic analysis of early murine mammary gland development using novel probe-level algorithms Stephen R Master Alexander J Stoddard L Charles Bailey Tien-Chi Pan Katherine D Dugan and Lewis A Chodosh Addresses Department of Cancer Biology University of Pennsylvania School of Medicine Philadelphia PA 19104-6160 USA. ỶDepartment of Pathology and Laboratory Medicine University of Pennsylvania School of Medicine Philadelphia PA 19104-6160 USA. Department of Medicine University of Pennsylvania School of Medicine Philadelphia PA 19104-6160 USA. Abramson Family Cancer Research Institute University of Pennsylvania School of Medicine Philadelphia PA 19104-6160 USA. Department of Pediatrics Children s Hospital of Philadelphia Philadelphia PA 19104 USA. Correspondence LewisAChodosh. E-mail chodosh@ Published I February 2005 Genome Biology 2005 6 R20 The electronic version of this article is the complete one and can be found online at http 2005 6 2 R20 Received 25 August 2004 Revised 1 October 2004 Accepted 8 December 2004 2005 Master et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We describe a novel algorithm ChipStat for detecting gene-expression changes utilizing probelevel comparisons of replicate Affymetrix oligonucleotide microarray data. A combined detection approach is shown to yield greater sensitivity than a number of widely used methodologies including SAM dChip and logit-T. Using this approach we identify alterations in functional pathways during murine neonatal-pubertal mammary development that include the coordinate upregulation of major urinary proteins and the downregulation of loci exhibiting reciprocal imprinting. Background The widespread use of
