Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Single-feature polymorphism discovery in the barley transcriptome. | Method Open Access Single-feature polymorphism discovery in the barley transcriptome Nils Rostoks Justin O Borevitz Peter E Hedley Joanne Russell Sharon Mudie Jenny Morris Linda Cardle David F Marshall and Robbie Waugh Addresses Scottish Crop Research Institute Genome Dynamics Invergowrie Dundee DD2 5DA Scotland UK. ỶUniversity of Chicago Department of Ecology and Evolution Chicago IL 60637 USA. Correspondence Justin O Borevitz. E-mail borevitz@. Robbie Waugh. E-mail rwaugh@ Published II May 2005 Genome Biology 2005 6 R54 doi 186 gb-2005-6-6-r54 The electronic version of this article is the complete one and can be found online at http 2005 6 6 R54 Received 8 February 2005 Revised 22 March 2005 Accepted I4 April 2005 2005 Rostoks et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10 000 single-feature polymorphisms SFP between two barley genotypes. The method has good sensitivity as 67 of known single-nucleotide polymorphisms SNP were called as SFPs. This method is applicable to all oligonucleotide microarray data accounts for SNP effects in geneexpression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes. Background Whole-genome sequences of Arabidopsis and rice have provided a fundamental platform for the discovery of gene content and function in dicot and monocot plants. Research on the model species has provided a wealth of knowledge on universal biochemical and genetic processes as well as the development of analytical tools that are applicable to other plant species 1-3 . The availability
