Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Chipper: discovering transcription-factor targets from chromatin immunoprecipitation microarrays using variance stabilization. | Mfethbd Open Access Chipper discovering transcription-factor targets from chromatin immunoprecipitation microarrays using variance stabilization Francis D Gibbons Markus Proft Kevin Struhl and Frederick P Roth Addresses Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Longwood Avenue Boston MA 02115 USA. ỶInstituto de Biología Molecular y Celular de Plantas IBMCP Universidad Politécnica de Valencia Camino de Vera s n 46022 Valencia Spain. Correspondence Frederick P Roth. E-mail fritz_roth@ Published I November 2005 Genome Biology 2005 6 R96 doi I86 gb-2005-6-I I-r96 The electronic version of this article is the complete one and can be found online at http 2005 6 II R96 Received 23 March 2005 Revised I August 2005 Accepted 30 September 2005 2005 Gibbons et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Chromatin immunoprecipitation combined with microarray technology Chip2 allows genomewide determination of protein-DNA binding sites. The current standard method for analyzing Chip2 data requires additional control experiments that are subject to systematic error. We developed methods to assess significance using variance stabilization learning error-model parameters without external control experiments. The method was validated experimentally shows greater sensitivity than the current standard method and incorporates false-discovery rate analysis. The corresponding software Chipper is freely available. The method described here should help reveal an organism s transcription-regulatory wiring diagram . Background A major goal in understanding cellular behavior is to reveal the wiring of transcriptional regulation through which transcription
