Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Challenging the spliceosome machine. | Research Challenging the spliceosome machine Michael Weir Matthew Eaton and Michael Rice Open Access Addresses Department of Biology Wesleyan University Middletown CT 06459 USA. ỶDepartment of Mathematics and Computer Science Wesleyan University Middletown CT 06459 USA. Correspondence Michael Weir. Email mweir@ Published 17 January 2006 Genome Biology 2006 7 R3 doi gb-2006-7-1-r3 The electronic version of this article is the complete one and can be found online at http 2006 7 1 R3 Received 15 September 2005 Revised 7 November 2005 Accepted 15 December 2005 2006 Weir et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Using cDNA copies of transcripts and corresponding genomic sequences from the Berkeley Drosophila Genome Project a set of 24 753 donor and acceptor splice sites were computed with a scanning algorithm that tested for single nucleotide insertion deletion and substitution polymorphisms. Using this dataset we developed a progressive partitioning approach to examining the effects of challenging the spliceosome system. Results Our analysis shows that information content increases near splice sites flanking progressively longer introns and exons suggesting that longer splice elements require stronger binding of spliceosome components. Information also increases at splice sites near very short introns and exons suggesting that short splice elements have crowding problems. We observe that the information found at individual splice sites depends upon a balance of splice element lengths in the vicinity including both flanking and non-adjacent introns and exons. Conclusion These results suggest an interdependence of multiple splicing events along the pre-mRNA .
