Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals. | Research Open Access RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals Mohammad Ali Faghihi and Claes Wahlestedt Addresses Department of Biochemistry The Scripps Research Institute 5353 Parkside Drive Jupiter FL 33458 USA. ỶCenter for Genomics and Bioinformatics Karolinska Institutet Berzelius văg SE-171 77 Stockholm Sweden. Correspondence Claes Wahlestedt. Email clawah@ Published 9 May 2006 Genome Biology 2006 7 R38 doi 186 gb-2006-7-5-r38 The electronic version of this article is the complete one and can be found online at http 2006 7 5 R38 Received 3 October 2005 Revised 6 December 2005 Accepted 13 April 2006 2006 Faghihi and Wahlestedt licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Antisense transcription yielding both coding and non-coding RNA is a widespread phenomenon in mammals. The mechanism by which natural antisense transcripts NAT may regulate gene expression are largely unknown. The aim of the present study was to explore the mechanism of reciprocal sense-antisense S-AS regulation by studying the effects of a coding and non-coding NAT on corresponding gene expression and to investigate the possible involvement of endogenous RNA interference RNAi in S-AS interactions. Results We have examined the mechanism of S-AS RNA base pairing using thymidylate synthase and hypoxia inducible factor-1a as primary examples of endogenous genes with coding and noncoding NAT partners respectively. Here we provide direct evidence against S-AS RNA duplex formation in the cytoplasm of human cells and subsequent activation of RNAi. Conclusion Collectively our data demonstrate that NAT regulation of gene expression occurs .
