Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The subcellular localization of the mammalian proteome comes a fraction closer. | Minireview The subcellular localization of the mammalian proteome comes a fraction closer Jeremy C Simpson and Rainer Pepperkok Address Cell Biology and Biophysics Unit European Molecular Biology Laboratory Meyerhofstrasse 1 69117 Heidelberg Germany. Correspondence Jeremy C Simpson. Email simpson@ Published 23 June 2006 Genome Biology 2006 7 222 doi gb-2006-7-6-222 The electronic version of this article is the complete one and can be found online at http 2006 7 6 222 2006 BioMed Central Ltd Abstract Another step along the road towards determining the subcellular localization of a complete mammalian proteome has been taken with a study using cellular fractionation and protein correlation profiling to identify and localize organellar proteins. Here we discuss this new work in the context of other strategies for large-scale subcellular localization. The landmark achievements of the complete sequencing of the human and mouse genomes are becoming a distant memory. Their importance has rightly been lauded but the use of these resources to gain a comprehensive understanding of the human proteome at a functional level has only just started. The identification of all potential open reading frames ORFs is doubtless the minimum information required to study the proteome and is an essential prerequisite to contemporary functional genomics and systems biology approaches. In this context one logical step towards our understanding of the proteome is the global determination of subcellular protein localization and how it may change for example as a result of extracellular stimuli or during development. Despite many parallel and complementary efforts this goal has still not been achieved for any mammalian proteome. Tag and tell On the face of things this may seem somewhat surprising as the localizome for the budding yeast Saccharomyces cere-visiae was reported back in 2003 1 effectively as a consequence of the availability of the yeast genome .
