Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Slow, stochastic transgene repression with properties of a timer. | Research Open Access Slow stochastic transgene repression with properties of a timer Clifford L Wang Desirée C Yang and Matthias Wabl Address Department of Microbiology and Immunology University of California San Francisco CA 94143-0414 USA. Correspondence CliffordLWang. Email Published 9 June 2006 Genome Biology 2006 7 R47 doi 186 gb-2006-7-6-r47 The electronic version of this article is the complete one and can be found online at http 2006 7 6 R47 Received 13 February 2006 Revised 30 March 2006 Accepted 8 May 2006 2006 Wang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background When gene expression varies unpredictably between genetically identical organisms this is sometimes ascribed as stochastic. With the prevalence of retroviral vectors stochastic repression is often observed and can complicate the interpretation of outcomes. But it may also faithfully reflect characteristics of sites in the genome. Results We created and identified several cell clones in which within a given cell retroviral transcription of a transgene was repressed heritably and essentially irreversibly. This repression was relatively slow total repression in all cells took months. We observed the dynamics of repression and found that they were ergodic that is tending with a probability to a final state independent of previous conditions. Different positions of the transgene in the genome demonstrated different dynamics. At a position on mouse chromosome 9 repression abided by near perfect first-order kinetics and was highly reproducible even under conditions where the number of cell generations per day varied. Conclusion We propose that such a cell division independent off mechanism .
