Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Comparative sequence analysis reveals an intricate network among REST, CREB and miRNA in mediating neuronal gene expression. | Research Open Access Comparative sequence analysis reveals an intricate network among REST CREB and miRNA in mediating neuronal gene expression Jie Wu and Xiaohui Xie Addresses Department of Biomedical Engineering Boston University Boston Massachusetts 02215 USA. ỶBroad Institute of MIT and Harvard 7 Cambridge Center Cambridge Massachusetts 02142 USA. Correspondence Xiaohui Xie. Email xhx@ Published 26 September 2006 Genome Biology 2006 7 R85 doi 186 gb-2006-7-9-r85 The electronic version of this article is the complete one and can be found online at http 2006 7 9 R85 Received 12 May 2006 Revised 1 August 2006 Accepted 26 September 2006 2006 Wu and Xie licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Two distinct classes of regulators have been implicated in regulating neuronal gene expression and mediating neuronal identity transcription factors such as REST NRSF RE1 silencing transcription factor and CREB cAMP response element-binding protein and microRNAs miRNAs . How these two classes of regulators act together to mediate neuronal gene expression is unclear. Results Using comparative sequence analysis here we report the identification of 895 sites NRSE as the putative targets of REST. A set of the identified NRSE sites is present in the vicinity of the miRNA genes that are specifically expressed in brain-related tissues suggesting the transcriptional regulation of these miRNAs by REST. We have further identified target genes of these miRNAs and discovered that REST and its cofactor complex are targets of multiple brain-related miRNAs including miR-124a miR-9 and miR-132. Given the role of both REST and miRNA as repressors these findings point to a double-negative .