Báo cáo sinh học: "Retroviral vectors encoding ADA regulatory locus control region provide enhanced T-cell-specific transgene expression"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Retroviral vectors encoding ADA regulatory locus control region provide enhanced T-cell-specific transgene expression | Genetic Vaccines and Therapy BioMed Central Research Retroviral vectors encoding ADA regulatory locus control region provide enhanced T-cell-specific transgene expression Alice T Trinh1 Bret G Ball2 Erin Weber3 Timothy K Gallaher1 Zoya Gluzman-Poltorak1 French Anderson1 and Lena A Basile 1 Open Access Address 1Neumedicines Inc Pasadena California 91107 USA 2Department of Neurosurgery Mayo Clinic Rochester Minnesota 55905 USA and 3Department of Surgery Harbor-UCLA Medical Center Torrance California 90502 USA Email Alice T Trinh - atrinh@ Bret G Ball - Erin Weber - erinwebe@ Timothy K Gallaher - gallaher@ Zoya Gluzman-Poltorak - zoya@ French Anderson - kathyanderson99@ Lena A Basile - basile@ Corresponding author Published 30 December 2009 Received 8 April 2009 T . rr Accepted 30 December 2009 Genetic Vaccines and Therapy 2009 7 13 doi 1479-0556-7-13 This article is available from http content 7 1 13 2009 Trinh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Murine retroviral vectors have been used in several hundred gene therapy clinical trials but have fallen out of favor for a number of reasons. One issue is that gene expression from viral or internal promoters is highly variable and essentially unregulated. Moreover with retroviral vectors gene expression is usually silenced over time. Mammalian genes in contrast are characterized by highly regulated precise levels of expression in both a temporal and a cell-specific manner. To ascertain if recapitulation of endogenous adenosine deaminase ADA expression can be achieved in a vector construct we created a new series of .