Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta | Genet Sei Evol 1990 22 57-64 Elsevier INRA 57 Original article Molecular cloning and partial sequence characterization of the duplicated amylase genes from Drosophila erecta L Bally-Cuif V Payant s Abukashawa BF Benkel DA Hickey Department of Biology University of Ottawa Ottawa Ontario KIN 6N5 Canada Received 16 May 1989 accepted 15 November 1989 Summary - Previous studies have shown that the a-amylase gene is duplicated in each of the 8 species comprising the Drosophila melanogaster specie subgroup. In this study a genomic library in phage lambda was prepared from the DNA of 1 species member of this subgroup D erecta. This gene library was screened with an amylase-specific probe from the closely-related species D melanogaster One of the cross-hybridizing phage clones was isolated insert fragments were sub-cloned into plasmid vectors and the identity of the cloned locus was verified by DNA sequencing. The results confirm that this species contains 2 very similar copies of the amylase-coding sequence as does D melanogaster. Within the amylase-coding region there is a 3 sequence divergence between the 2 species this value rises to 6 if we consider only silent sites within the coding region. Sequence divergence between D erecta and D melanogaster in the non-coding intergenic region is approximately 15 . These results constitute the first molecular sequence data available for protein-coding genes of D erecta. The data set allows us to calculate an estimated divergence time of more than 10 million years between the 2 species. This is consistent with the results of previous phylogenetic studies. Drosophila erecta I a-amylase molecular evolution gene conversion Resume Clonage et caractérisation d une sequence partielle des gènes dupliqués de 1 amylase chez Drosophila erecta - Des etudes antérieures ont montré que le gène a-amylase étaự dupliqụé chez chacune des 8 espèces du sous-groupe Drosophila melanogaster. Dans la présẹnte etude une banque génọmique a été préparée .
