Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes. | Open Access Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes Michael Xavier Doss Johannes Winkler Shuhua Chen Rita Hippler-Altenburg Isaia Sotiriadou Marcel Halbach Kurt Pfannkuche Huamin Liang Herbert Schulz Oliver Hummed Norbert Hubner Ruth Rottscheidt Jurgen Hescheler and Agapios Sachinidis Addresses Center of Physiology and Pathophysiology Institute of Neurophysiology University of Cologne Robert Koch Str. 50931 Cologne Germany. tMax-Delbrueck-Center for Molecular Medicine - MDC Robert-Rossle Str. 13092 Berlin Germany. institute for Genetics Department of Evolutionary Genetics University of Cologne Zulpicher Str. 50674 Cologne Germany. Correspondence Agapios Sachinidis. Email Published II April 2007 Genome Biology 2007 8 R56 doi I86 gb-2007-8-4-r56 The electronic version of this article is the complete one and can be found online at http 2007 8 4 R56 Received I8 December 2006 Revised I6 February 2007 Accepted II April 2007 2007 Doss et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Characterization of gene expression signatures for cardiomyocytes derived from embryonic stem cells will help to define their early biologic processes. Results A transgenic a-myosin heavy chain MHC embryonic stem cell lineage was generated exhibiting puromycin resistance and expressing enhanced green fluorescent protein EGFP under the control of the a-MHC promoter. A puromycin-resistant EGFP-positive a-MHC-positive cardiomyocyte population was isolated with over 92 purity. RNA was isolated after electrophysiological characterization of the cardiomyocytes. Comprehensive transcriptome analysis of a-MHC-positive cardiomyocytes in .
