Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of CDK2 substrates in human cell lysates. | Open Access Research Identification of CDK2 substrates in human cell lysates Yong Chi Markus Welcker Asli A Hizli Jeffrey J Posakony Ruedi Aebersold and Bruce E Clurman Addresses Divisions of Clinical Research and Human Biology Fred Hutchinson Cancer Research Center 1100 Fairview Avenue N. Seattle WA 98109 USA. Institute for Systems Biology 1441 N. 34th Street Seattle WA 98103 USA. Division of Basic Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Avenue N. Seattle WA 98109 USA. institute of Molecular Systems Biology ETH Zurich and Faculty of Science University of Zurich 8093 Zurich Switzerland. Correspondence Bruce E Clurman. Email bclurman@ Published 13 October 2008 Genome Biology 2008 9 R149 doi gb-2008-9- 10-r 149 The electronic version of this article is the complete one and can be found online at http 2008 9 10 R149 Received 8 August 2008 Revised 29 September 2008 Accepted l3 October 2008 2008 Chi et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Protein phosphorylation regulates a multitude of biological processes. However the large number of protein kinases and their substrates generates an enormously complex phosphoproteome. The cyclin-dependent kinases - the CDKs - comprise a class of enzymes that regulate cell cycle progression and play important roles in tumorigenesis. However despite intense study only a limited number of mammalian CDK substrates are known. A comprehensive understanding of CDK function requires the identification of their substrate network. Results We describe a simple and efficient approach to identify potential cyclin A-CDK2 targets in complex cell lysates. Using a kinase engineering strategy combined with chemical .
