Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Characterization and comparative profiling of the small RNA transcriptomes in two phases of locust. | Open Access Researc h Characterization and comparative profiling of the small RNA transcriptomes in two phases of locust Yuanyuan Wei Shuang Chen Pengcheng Yang Zongyuan Ma and Le Kang Address State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 PR China. Correspondence Le Kang. Email lkang@ Published 16 January 2009 Genome Biology 2009 10 R6 doi gb-2009-l 0-l-r6 The electronic version of this article is the complete one and can be found online at http 2009 l0 l R6 Received 28 September 2008 Revised ll December 2008 Accepted l6 January 2009 2009 Wei et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background All the reports on insect small RNAs come from holometabolous insects whose genome sequence data are available. Therefore study of hemimetabolous insect small RNAs could provide more insights into evolution and function of small RNAs in insects. The locust is an important economically harmful hemimetabolous insect. Its phase changes as a phenotypic plasticity result from differential gene expression potentially regulated at both the post-transcriptional level mediated by small RNAs and the transcriptional level. Results Here using high-throughput sequencing we characterize the small RNA transcriptome in the locust. We identified 50 conserved microRNA families by similarity searching against miRBase and a maximum of l85 potential locust-specific microRNA family candidates were identified using our newly developed method independent of locust genome sequence. We also demonstrate conservation of microRNA and evolutionary analysis of locust microRNAs indicates .
