Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology. | Open Access Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology Tao Liu David Sims and Buzz Baum Addresses MRC Laboratory of Molecular Cell Biology UCL Gower Street London WC1E 6BT UK. The Institute of Cancer Research Chester Beatty Laboratories Fulham Road London SW3 6JB UK. Correspondence Buzz Baum. Email Published 5 March 2009 Genome Biology 2009 10 R26 doi gb-2009- l0-3-r26 The electronic version of this article is the complete one and can be found online at http 2009 l0 3 R26 Received 27 November 2008 Revised 18 February 2009 Accepted 5 March 2009 2009 Liu et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In recent years RNAi screening has proven a powerful tool for dissecting gene functions in animal cells in culture. However to date most RNAi screens have been performed in a single cell line and results then extrapolated across cell types and systems. Results Here to dissect generic and cell type-specific mechanisms underlying cell morphology we have performed identical kinome RNAi screens in six different Drosophila cell lines derived from two distinct tissues of origin. This analysis identified a core set of kinases required for normal cell morphology in all lines tested together with a number of kinases with cell type-specific functions. Most significantly the screen identified a role for minibrain mnb DYRKlA a kinase associated with Down s syndrome in the regulation of actin-based protrusions in CNS-derived cell lines. This cell type-specific requirement was not due to the peculiarities in the morphology of CNS-derived cells and could not be attributed to .
