Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: A computational approach for genome-wide mapping of splicing factor binding sites. | Open Access Method A computational approach for genome-wide mapping of splicing factor binding sites Martin Akerman Hilda David-Eden Ron Y Pinter and Yael Mandel-Gutfreund Addresses Department of Biology the Technion - Israel Institute of Technology Haifa 32000 Israel. Department of Computer Science Technion - Israel Institute of Technology Haifa 32000 Israel. Correspondence Yael Mandel-Gutfreund. Email yaelmg@ Published 18 March 2009 Genome Biology 2009 10 R30 doi gb-2009- 10-3-r30 The electronic version of this article is the complete one and can be found online at http 2009 10 3 R30 Received 18 December 2008 Revised 26 February 2009 Accepted 18 March 2009 2009 Akerman et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Alternative splicing is regulated by splicing factors that serve as positive or negative effectors interacting with regulatory elements along exons and introns. Here we present a novel computational method for genome-wide mapping of splicing factor binding sites that considers both the genomic environment and the evolutionary conservation of the regulatory elements. The method was applied to study the regulation of different alternative splicing events uncovering an interesting network of interactions among splicing factors. Background Alternative splicing AS is a post-transcriptional process responsible for producing distinct protein isoforms as well as down-regulation of translation. Many experimental and computational studies revealed that AS can be regulated in a tissue-specific manner 1-4 during embryonic development 5 or in response to particular cellular stimuli 6 . AS regulation is known to be mediated by many splicing factors SFs generally .
