Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: A whole-genome assembly of the domestic cow, Bos taurus. | Researc h Open Access A whole-genome assembly of the domestic cow Bos taurus Aleksey V Zimin Arthur L DelcheP Liliana Florea David R Kelley Michael C Schatz Daniela Puiu Finnian Hanrahan Geo Pertea Curtis P Van Tassell Tad S Sonstegard Guillaume Marẹais Michael Roberts Poorani Subramanian James A Yorke and Steven L Salzberg Addresses institute for Physical Science and Technology University of Maryland College Park Maryland 20742 USA. Center for Bioinformatics and Computational Biology University of Maryland College Park Maryland 20742 USA. Agricultural Research Service . Department of Agriculture 10300 Baltimore Ave. Beltsville Maryland 20705 USA. Correspondence Steven L Salzberg. Email salzberg@ Published 24 April 2009 Genome Biology 2009 I0 R42 doi gb-2009- 10-4-r42 The electronic version of this article is the complete one and can be found online at http 2009 10 4 R42 Received 7 January 2009 Revised 6 February 2009 Accepted 24 April 2009 2009 Zimin et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The genome of the domestic cow Bos taurus was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods. Results We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques creating an assembly of billion base pairs that has multiple improvements over previous assemblies it is more complete covering more of the genome thousands of gaps have been closed many erroneous inversions deletions and translocations have been corrected and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more .
