Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Induced pluripotent stem cells free of exogenous reprogramming factors. | Minireview Induced pluripotent stem cells free of exogenous reprogramming factors Ramzey Abujarour and Sheng Ding Address Department of Chemistry The Scripps Research Institute 10550 North Torrey Pines Road La Jolla CA 92037 USA. Correspondence Sheng Ding. Email sding@ Published 6 May 2009 Genome Biology 2009 10 220 doi gb-2009-10-5-220 The electronic version of this article is the complete one and can be found online at http 2009 10 5 220 2009 BioMed Central Ltd Abstract The development of novel approaches for reprogramming mouse and human somatic cells has enabled the generation of induced pluripotent stem cells that are free of exogenous genes. The epigenome of differentiated somatic cells can be reprogrammed to a pluripotent state by nuclear transfer into enucleated oocytes or by fusion with pluripotent cells such as embryonic stem cells ESCs 1 . More recently it has been shown that overexpression of defined transcription factors via transduction of viral vectors can reprogram mouse and human somatic cells to induced pluripotent stem cells iPSCs 2-4 . This new approach greatly simplifies the generation of pluripotent cells bypassing many technical and ethical hurdles and brings closer the possibility of using patient-specific cells in cell-based therapy. However the use of viruses to deliver the reprogramming factors entails permanent genetic alterations that render the cells inappropriate for many in vitro and in vivo applications. Several approaches have recently been devised to generate iPSCs free of the exogenous reprogramming factor genes including the use of non-integrating approaches for transgene delivery 5 6 . Four papers published this year describe a variety of novel approaches. Soldner et al. 7 have used the Cre loxP recombination system to produce human iPSCs free of exogenous reprogramming genes. Woltjen et al. 8 and Kaji et al. 9 demonstrate that the piggyBac PB transposon system can be used both to introduce
