Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during | Open Access Dynamic expression of small non-coding RNAs including novel microRNAs and piRNAs 21U-RNAs during Caenorhabditis elegans development Masaomi Kato Alexandre de Lencastre Zachary Pincus and Frank J Slack Address Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520 USA. Correspondence Frank J Slack. Email Published 21 May 2009 Genome Biology 2009 10 R54 doi gb-2009- l0-5-r54 The electronic version of this article is the complete one and can be found online at http 2009 l0 5 R54 Received 30 January 2009 Revised 28 April 2009 Accepted 21 May 2009 2009 Kato et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Small non-coding RNAs including microRNAs miRNAs serve an important role in controlling gene expression during development and disease. However little detailed information exists concerning the relative expression patterns of small RNAs during development of animals such as Caenorhabditis elegans. Results We performed a deep analysis of small RNA expression in C. elegans using recent advances in sequencing technology and found that a significant number of known miRNAs showed major changes in expression during development and between males and hermaphrodites. Additionally we identified 66 novel miRNA candidates about 35 of which showed transcripts from their star sequence suggesting that they are bona fide miRNAs. Also hundreds of novel Piwi-interacting RNAs piRNAs 21U-RNAs with dynamic expression during development together with many longer transcripts encompassing 21U-RNA sequences were detected in our libraries. Conclusions Our analysis reveals extensive regulation of non-coding small RNAs during .
