Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. †Frank M. Ryburn Jr. Cardiology Center, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. ‡Center for Biomedical Inventions, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. §Department of Biochemistry, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. ¶Department of Internal Medicine, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA. Correspondence: Jeoffrey J Schageman | Software Open Access ELXR a resource for rapid exon-directed sequence analysis Jeoffrey J Schageman Christopher J Horton Sijing Niu Harold R Garner and Alexander Pertsemlidis Addresses Eugene McDermott Center for Human Growth and Development University of Texas Southwestern Medical Center Harry Hines Boulevard Dallas TX 75390 USA. Frank M. Ryburn Jr. Cardiology Center University of Texas Southwestern Medical Center Harry Hines Boulevard Dallas TX 75390 USA. Center for Biomedical Inventions University of Texas Southwestern Medical Center Harry Hines Boulevard Dallas TX 75390 USA. Department of Biochemistry University of Texas Southwestern Medical Center Harry Hines Boulevard Dallas TX 75390 USA. Department of Internal Medicine University of Texas Southwestern Medical Center Harry Hines Boulevard Dallas TX 75390 USA. Correspondence JeoffreyJ Schageman. E-mail Published 28 April 2004 Genome Biology 2004 5 R36 The electronic version of this article is the complete one and can be found online at http 2004 5 5 R36 Received 8 January 2004 Revised 13 February 2004 Accepted 14 April 2004 2004 Schageman et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract ELXR Exon Locator and Extractor for Resequencing streamlines the process of determining exon intron boundaries and designing PCR and sequencing primers for high-throughput resequencing of exons. We have pre-computed ELXR primer sets for all exons identified from the human mouse and rat mRNA reference sequence RefSeq public databases curated by the National Center for Biotechnology Information. The resulting exon-flanking PCR primer pairs have been compiled into a system called ELXRdb which may be searched by keyword gene name or RefSeq accession number. Rationale With the vast amount .
