Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Assisted assembly: how to improve a de novo genome assembly by using related species. | Open Access Method Assisted assembly how to improve a de novo genome assembly by using related species Sante Gnerre Eric S Lander Kerstin Lindblad-Toh and David B Jaffe Addresses Broad Institute of Harvard and MIT Cambridge Center Cambridge Massachusetts 02142 USA. Department of Medical Biochemistry and Microbiology Uppsala University Uppsala 751 23 Sweden. Correspondence Sante Gnerre. Email sante@ Published 27 August 2009 Genome Biology 2009 10 R88 doi gb-2009- l0-8-r88 The electronic version of this article is the complete one and can be found online at http 2009 10 8 R88 Received 7 April 2009 Revised 8 July 2009 Accepted 27 August 2009 2009 Gnerre et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We describe a new assembly algorithm where a genome assembly with low sequence coverage either throughout the genome or locally due to cloning bias is considerably improved through an assisting process via a related genome. We show that the information provided by aligning the whole-genome shotgun reads of the target against a reference genome can be used to substantially improve the quality of the resulting assembly. Background How completely one can reconstruct a genome sequence from whole-genome shotgun WGS reads depends on the depth of sequence coverage generated 1 . Additionally longer reads and better base quality in reads provides more information and therefore allows any assembler to perform a better task resulting in both the generation of bigger contigs scaffolds and improvements in the quality of the assembly. The genomes of many species including the mammals Mus mus-culus 2 Canis familiaris 3 and Monodelphis domestica 4 have been assembled from .
