Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Nucleosome deposition and DNA methylation at coding region boundaries. | Open Access Nucleosome deposition and DNA methylation at coding region boundaries Jung Kyoon Choi Jae-Bum Bae Jaemyun Lyu Tae-Yoon Kim and Young-Joon Kim Addresses Department of Biochemistry College of Life Science and Technology Yonsei University 134 Sinchon-dong Seodaemun-gu Seoul Korea. Taboratoiy of Dermato-Immunology The Catholic University of Korea 505 Banpo-dong Seocho-gu Seoul Korea. Correspondence Young-Joon Kim. Email yjkim@ Published I September 2009 Genome Biology 2009 10 R89 doi gb-2009- I0-9-r89 The electronic version of this article is the complete one and can be found online at http 2009 I0 9 R89 Received 24 June 2009 Revised I0 August 2009 Accepted I September 2009 2009 Choi et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Nucleosome deposition downstream of transcription initiation and DNA methylation in the gene body suggest that control of transcription elongation is a key aspect of epigenetic regulation. Results Here we report a genome-wide observation of distinct peaks of nucleosomes and methylation at both ends of a protein coding unit. Elongating polymerases tend to pause near both coding ends immediately upstream of the epigenetic peaks causing a significant reduction in elongation efficiency. Conserved features in underlying protein coding sequences seem to dictate their evolutionary conservation across multiple species. The nucleosomal and methylation marks are commonly associated with high sequence-encoded DNA-bending propensity but differentially with CpG density. As the gene grows longer the epigenetic codes seem to be shifted from variable inner sequences toward boundary regions rendering the peaks more prominent in higher .
