Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: A global survey identifies novel upstream components of the Ath5 neurogenic network. | Open Access Researc h A global survey identifies novel upstream components of the Ath5 neurogenic network Marcel Souren Juan Ramon Martinez-Morales Panagiota Makri Beate Wittbrodt and Joachim Wittbrodt Addresses Developmental Biology Unit EMBL-Heidelberg Meyerhofstrasse Heidelberg 69117 Germany. Centro Andaluz de Biología del Desarrollo CABD CSIC-Universidad Pablo de Olavide Carretera de Utrera Km1 Sevilla 41013 Spain. H These authors contributed equally to this work. Correspondence Juan Ramon Martinez-Morales. Email jrmarmor@. Joachim Wittbrodt. Email Published 7 September 2009 Genome Biology 2009 10 R92 doi gb-2009- 10-9-r92 The electronic version of this article is the complete one and can be found online at http 2009 10 9 R92 Received 14 April 2009 Revised 29 July 2009 Accepted 7 September 2009 2009 Souren et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Investigating the architecture of gene regulatory networks GRNs is essential to decipher the logic of developmental programs during embryogenesis. In this study we present an upstream survey approach termed trans-regulation screen to comprehensively identify the regulatory input converging on endogenous regulatory sequences. Results Our dual luciferase-based screen queries transcriptome-scale collections of cDNAs. Using this approach we study the regulation of Ath5 the central node in the GRN controlling retinal ganglion cell RGC specification in vertebrates. The Ath5 promoter integrates the input of upstream regulators to enable the transient activation of the gene which is an essential step for RGC differentiation. We efficiently identified potential Ath5 regulators that were
