Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia. | Open Access Researc h Preferential binding of HIF-I to transcriptionally active loci determines cell-type specific response to hypoxia Xiaobo Xia and Andrew L Kung Address Department of Pediatric Oncology Dana-Farber Cancer Institute Children s Hospital Boston and Harvard Medical School Binney Street Boston MA 02115 USA. Correspondence Andrew L Kung. Email andrew_kung@ Published 14 October 2009 Genome Biology 2009 10 doi gb-2009-10-10-r113 The electronic version of this article is the complete one and can be found online at http 2009 10 10 R113 Received 11 June 2009 Revised 18 September 2009 Accepted 14 October 2009 2009 Xia and Kung licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hypoxia-inducible factor 1 HIF-1 plays a key role in cellular adaptation to hypoxia. To better understand the determinants of HIF-1 binding and transactivation we used ChIP-chip and gene expression profiling to define the relationship between the epigenetic landscape sites of HIF-1 binding and genes transactivated by hypoxia in two cell lines. Results We found that when cells were acutely subjected to hypoxia HIF-1 preferentially bound to loci that were already transcriptionally active under normal growth conditions characterized by the presence of histone H3 lysine 4 methylation the presence of RNA polymerase II and basal production of mRNA. Cell type-specific differences in HIF-1 binding were largely attributable to differences in the basal gene expression patterns in the cells prior to the onset of hypoxia. Conclusions These results suggest that the repertoire of genes active in a cell for example through lineage specific transcription factors defines the subset of genes that .
