Báo cáo y học: "Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox. | Research Open Access Archaeal phylogeny based on proteins of the transcription and translation machineries tackling the Methanopyrus kandleri paradox Céline Brochier Patrick Forterre and Simonetta Gribaldo Addresses Equipe Phylogénomique Université Aix-Marseille I Centre Saint-Charles 13331 Marseille Cedex 3 France. fInstitut de Génétique et Microbiologie CNRS UMR 8621 Université Paris-Sud 91405 Orsay France. Correspondence Céline Brochier. E-mail Published 26 February 2004 Genome Biology 2004 5 R17 The electronic version of this article is the complete one and can be found online at http 2004 5 3 R17 Received 14 November 2003 Revised 5 January 2004 Accepted 21 January 2004 2004 Brochier et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Phylogenetic analysis of the Archaea has been mainly established by 16S rRNA sequence comparison. With the accumulation of completely sequenced genomes it is now possible to test alternative approaches by using large sequence datasets. We analyzed archaeal phylogeny using two concatenated datasets consisting of 14 proteins involved in transcription and 53 ribosomal proteins 3 275 and 6 377 positions respectively . Results Important relationships were confirmed notably the dichotomy of the archaeal domain as represented by the Crenarchaeota and Euryarchaeota the sister grouping of Sulfolobales and Aeropyrum pernix and the monophyly of a large group comprising Thermoplasmatales Archaeoglobus fulgidus Methanosarcinales and Halobacteriales with the latter two orders forming a robust cluster. The main difference concerned the position of Methanopyrus kandleri which grouped with Methanococcales and Methanobacteriales in the translation tree whereas it emerged at the base of the .

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