Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-wide insights into eukaryotic transcriptional control. | Goodrich and Kugel Genome Biology 2010 11 305 http 2010 11 6 305 w Genome Biology MEETING REPORT L Genome-wide insights into eukaryotic transcriptional control James A Goodrich and Jennifer F Kugel Abstract A report of the Keystone Symposium on Dynamics of Eukaryotic Transcription during Development Big Sky Montana USA 7-12 April 2010. The 2010 Keystone Symposium focusing on mechanisms of eukaryotic transcriptional regulation featured a strong emphasis on genomic approaches. Many presentations included data that coupled chromatin immunoprecipitation ChIP assays to deep sequencing ChIP-seq or tiling arrays ChIP-chip in order to map the locations of transcription factors and RNA polymerase II Pol II across the genomes of organisms from yeast to humans. In addition biochemical techniques such as permanganate footprinting nuclear run-on and nuclease digestion were applied to cellular chromatin and coupled to deep sequencing. Here we group the presentations that focused primarily on genomics into three general categories promoter-proximal paused polymerases chromatin and nucleosomes and networks of transcriptional regulation. Promoter-proximal paused polymerases Historically regulation of transcription was thought to occur primarily at the point of recruiting Pol II and its accessory factors to the promoters of genes. Now a battery of genomic studies is revealing that transcriptional regulation occurs at a post-initiation step at thousands of genes in both Drosophila and mammals. The signature of such genes is the presence of a promoter-proximal paused Pol II molecule - one that has initiated transcription and is poised for an activation signal in order to continue transcribing. Rick Young Whitehead Institute and Massachusetts Institute of Technology Cambridge USA in his keynote address provided a mechanism for how activation of paused polymerases Correspondence Department of .
