Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencing. | Zhao et al. Genome Biology 2010 11 R114 http 2010 11 11 R114 Genome Biology RESEARCH Open Access Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencing 1t 2t 1t 3 34 QI Zl Ido EWcll F Kllkliess Otdvid L Cdbdllero redro A Gdldnte Rdpildel B rdll I liyidl II Lee Ldsdll F 1 1 I -s Z 4 ĩ r Z 4 F II z I I z z5 A I z rz T- D M r z r r I r r 6 D I f i D r 4 F z l r z I Z Z F I I 7 3 Sdmdnind KUdi Zhen Ye Sdmuel Levy And lerezd R Vdsconcelos Ding Ren Sdndro J de Souzd Andmdrid A Cdmdrgo3 Andrew JG Simpson 1 Robert L Strdusberg1 Abstract Background To identify potentidl tumor suppressor genes genome-wide Qdtd from exome dnQ trdnscriptome sequencing were combined to sedrch for genes with loss of heterozygosity dnd dllele-specific expression. The dndlysis wds conducted on the bredst cdncer cell line HCC1954 dnd d lymphobldst cell line from the sdme indiVidudl HCC1954BL. Results By compdring exome sequences from the two cell lines we identified loss of heterozygosity events dt 403 genes in HCC1954 dnd dt one gene in HCC1954BL. The combindtion of exome dnd trdnscriptome sequence ddtd dlso revedled 86 dnd 50 genes with dllele specific expression events in HCC1954 dnd HCC1954BL which comprise dnd of genes surveyed respectively. Mdny of these genes identified by loss of heterozygosity dnd dllele-specific expression dre known or putdtive tumor suppressor genes such ds BRCA1 MSH3 dnd SETX which pdrticipdte in DNA repdir pdthwdys. Conclusions Our results demonstrate thdt the combined dpplicdtion of high throughput sequencing to exome dnd dllele-specific trdnscriptome dndlysis cdn revedl genes with known tumor suppressor chdrdcteristics dnd d shortlist of novel cdndiddtes for the study of tumor suppressor dctivities. Background Cancer arises from the accumulation of genetic and epigenetic changes that disrupt the normal regulatory controls in cells. Recently next generation sequencing technology
