Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Defining potentially conserved RNA regulons of homologous zinc-finger RNA-binding proteins. | Scherrer et al. Genome Biology 2011 12 R3 http 2011 12 1 R3 w Genome Biology RESEARCH Open Access Defining potentially conserved RNA regulons of homologous zine-finger RNA-binding proteins Ci bdrrdr1 2 brid in l-dmmdr1 3 Hiocc4 Pl locli Adbdrcc ilc l4 p rCcurbdr1 Tanja Scherrer Christian remmei Ralph Sciuess Ruedi Aebersold Andre P Gerber Abstract Background Glucose inhibition of gluconeogenic growth suppressor 2 protein Gis2p and zinc-finger protein 9 ZNF9 are conserved yeast and human zinc-finger proteins. The function of yeast Gis2p is unknown but human ZNF9 has been reported to bind nucleic acids and mutations in the ZNF9 gene cause the neuromuscular disease myotonic dystrophy type 2. To explore the impact of these proteins on RNA regulation we undertook a systematic analysis of the RNA targets and of the global implications for gene expression. Results Hundreds of mRNAs were associated with Gis2p mainly coding for RNA processing factors chromatin modifiers and GTPases. Target mRNAs contained stretches of G A U A U trinucleotide repeats located in coding sequences which are sufficient for binding to both Gis2p and ZNF9 thus implying strong structural conservation. Predicted ZNF9 targets belong to the same functional categories as seen in yeast indicating functional conservation which is further supported by complementation of the large cell-size phenotype of gis2 mutants with ZNF9. We further applied a matched-sample proteome-transcriptome analysis suggesting that Gis2p differentially coordinates expression of RNA regulons primarily by reducing mRNA and protein levels of genes required for ribosome assembly and by selectively up-regulating protein levels of myosins. Conclusions This integrated systematic exploration of RNA targets for homologous RNA-binding proteins indicates an unexpectedly high conservation of the RNA-binding properties and of potential targets thus predicting conserved RNA regulons. We also predict regulation of .
