Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Single cell transcriptomics of neighboring hyphae of Aspergillus nige. | de Bekker et al. Genome Biology 2011 12 R71 http 2011 12 8 R71 Genome Biology METHOD Open Access Single cell transeriptomies of neighboring hyphae of Aspergillus niger 1 2 23 23 1 Charissa de Bekker Oskar Bruning Martijs J Jonker Timo M Breit and Han AB Wosten Abstract Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7 of the A. niger genes of which 12 showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories. Background Cellular heterogeneity within an isogenic cell population is a widespread event in both prokaryotic and eukaryotic organisms. Heterogeneity of cells can be beneficial for the organism in many ways. Many documented cases of phenotypic variability in microorganisms relate to responses to environmental stress. This suggests that phenotypic variation aids in the survival of cells under adverse conditions and therefore may be an evolvable trait 1 2 . It has been shown that mycelia of filamentous fungi are also heterogeneous. For instance protein secretion 3-5 and gene expression 6-9 are heterogeneous between zones of fungal colonies. These differences were explained by the availability of carbon source and by spatial and temporal differentiation 7 . Heterogeneous gene expression can even be found within a zone of a colony. In fact expression of the glucoamylase gene glaA the acid amylase gene aamA the a-glucuronidase gene aguA and the feruloyl esterase gene faeA is heterogeneous between neighboring hyphae at the periphery of the colony of Aspergillus niger 10 11 . Co-expression studies showed that hyphae that highly express one of these genes also highly express the other genes encoding secreted proteins 11 . Moreover these hyphae highly express the glyceraldehyde-3-phos-phate .
