Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages. | AIDS Research and Therapy BioMed Central Research Open Access Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34 progenitor cells and derivation of HIV-1 resistant T cells and macrophages Akhil Banerjea1 Ming-Jie Li2 Leila Remling1 John Rossi2 and Ramesh Akkina 1 Address 1Dept. Microbiology Immunology and Pathology Colorado State University Fort Collins Colorado 80523 USA and 2Division of Molecular Biology Beckman Research Institute of the City of Hope 1450 East Duarte Road Duarte California 91010 USA Email Akhil Banerjea - akhil@ Ming-Jie Li - mili@ Leila Remling - remling@ John Rossi - jrossi@ Ramesh Akkina - akkina@ Corresponding author Published 17 December 2004 Received 01 October 2004 Accepted 17 December 2004 AIDS Research and Therapy 2004 1 2 doi 1742-6405-1 -2 This article is available from http content 1 1 2 2004 Banerjea et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background RNA based antiviral approaches against HIV-1 are among the most promising for long-term gene therapy. These include ribozymes aptamers decoys and small interfering RNAs siRNAs . Lentiviral vectors are ideal for transduction of such inhibitory RNAs into hematopoietic stem cells due to their ability to transduce non-dividing cells and their relative refractiveness to gene silencing. The objective of this study is to introduce an HIV-1 Tar aptamer either alone or in combination with an anti-CCR5 ribozyme into CD34 hematopoietic progenitor cells via an HIVbased lentiviral vector to derive viral resistant progeny T cells and macrophages. Results High efficiency and sustained gene transfer into CD34 cells were achieved with .
