Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Human immunodeficiency virus type-1 episomal cDNA in semen. | BioMed Central AIDS Research and Therapy Research Open Access Human immunodeficiency virus type-1 episomal cDNA in semen Chong Xu 1 Joseph A Politch1 Kenneth H Mayer2 3 and Deborah J Anderson 1 Address 1Division of Reproductive Biology Department of Obstetrics and Gynecology Boston University School of Medicine Boston MA 02118 USA 2Fenway Community Health Center Boston MA 02115 USA and 3Department of Medicine Brown University Medical School Providence RI 02912 USA Email Chong Xu - Joseph A Politch - Kenneth H Mayer - kenneth_mayer@ Deborah J Anderson - Corresponding author Published II October 2005 Received 20 June 2005 AIDS Research and Therapy 2005 2 9 doi 1742-6405-2-9 Accepted 1 1 October 2005 This article is available from http content 2 I 9 2005 Xu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Episomal 2-long terminal repeat LTR HIV-1 cDNA a by-product of HIV-1 infection is used in clinical trials as a marker for ongoing viral replication. It would be useful to employ 2-LTR cDNA to monitor cryptic HIV-1 infection in the genital tract of men on antiretroviral therapy ART to predict the evolution of sexually transmissible drug-resistant HIV-1 but studies thus far have failed to detect this marker in semen. The objectives of this study were 1 to use a technique that maximizes DNA recovery from HIV-1 infected white blood cells in semen to determine if episomal 2-LTR cDNA is detectable in semen of ART-naive men with other evidence of genital tract HIV-1 infection and 2 to compare levels of HIV-1 2-LTR cDNA RNA and proviral DNA in semen from HIV-1 men on ART. Results Using a somatic cell DNA .
