Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells | Kim et al. Journal of Biomedical Science 2010 17 85 http content 17 1 85 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells 11 11 1 2 2 2 2 Ki Chan Kim Hyo Sang Go Hae Rang Bak Chang Soon Choi Inha Choi Pitna Kim Seol-Heui Han So Min Han1 Chan Young Shin2 Kwang Ho Ko1 Abstract Background Prenatal ethanol exposure during pregnancy induces a spectrum of mental and physical disorders called fetal alcohol spectrum disorder FASD . The central nervous system is the main organ influenced by FASD and neurological symptoms include mental retardation learning abnormalities hyperactivity and seizure susceptibility in childhood along with the microcephaly. In this study we examined whether ethanol exposure adversely affects the proliferation of NPC and de-regulates the normal ratio between glutamatergic and GABAergic neuronal differentiation using primary neural progenitor culture NPC and in vivo FASD models. Methods Neural progenitor cells were cultured from E14 embryo brain of Sprague-Dawley rat. Pregnant mice and rats were treated with ethanol 2 or 4 g kg day diluted with normal saline from E7 to E16 for in vivo FASD animal models. Expression level of proteins was investigated by western blot analysis and immunocytochemical assays. MTT was used for cell viability. Proliferative activity of NPCs was identified by BrdU incorporation immunocytochemistry and FACS analysis. Results Reduced proliferation of NPCs by ethanol was demonstrated using BrdU incorporation immunocytochemistry and FACS analysis. In addition ethanol induced the imbalance between glutamatergic and GABAergic neuronal differentiation via transient increase in the expression of Pax6 Ngn2 and NeuroD with concomitant decrease in the expression of Mash1. Similar pattern of .
