Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Role of taurine on acid secretion in the rat stomach | Huang et al. Journal of Biomedical Science 2011 18 11 http content 18 1 11 JOURNAL OF BIOMEDICAL SCIENCE The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan RESEARCH Open Access Role of taurine on acid secretion in the rat stomach Kai-Han Huang1 2 Chia-Chieh Chang 3 Jau-Der Ho1 2 Ruey-Hwa Lu4 Li Hsueh Tsai3 5 Abstract Background Taurine has chemical structure similar to an inhibitory neurotransmitter g-aminobutyric acid GABA . Previous studies on GABA in the stomach suggest GABAergic neuron is involved in acid secretion but the effects of taurine are poor understood. Methods The effects of taurine on acid secretion signal transduction and localization of taurinergic neurons were determined in the rat stomach using everted whole stomach RIA kit and immunohistochemical methods. Results We used antibodies against taurine-synthesizing enzyme cysteine sulfuric acid decarboxylase CSAD and taurine. CSAD- and taurine-positive cells were found in the muscle and mucosal layers. Distributions of CSAD- and taurine-positive cells in both mucosal and muscle layers were heterogeneous in the stomach. Taurine at 10-9 10-4 M induced acid secretion and the maximum secretion was at 10-5 M higher than the spontaneous secretion. Taurine-induced acid secretion was completely inhibited by bicuculline and atropine but not by cimetidine proglumide or strychnine. Atropine and tetrodotoxin TTX completely inhibited the acid secretion induced by low concentrations of taurine and partially inhibited induced by high concentrations. Verapamil a calcium blocker agent inhibited acid output elicited by taurine. We assumed all Ca2 channels involved in the response to these secretagogues were equally affected by verapamil. Intracellular cAMP adenosine 3 5 -monophosphat in the stomach significantly increased with taurine treatment in a dose-dependent manner. High correlation r p of taurine concentrations
