Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Fish-on-a-chip: a sensitive detection microfluidic system for alzheimer’s disease | Devadhasan et al. Journal of Biomedical Science 2011 18 33 http content 18 1 33 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE REVIEW Open Access Fish-on-a-chip a sensitive detection microfluidic system for alzheimer s disease Jasmine P Devadhasan 1 Sanghyo Kim1 and Jeongho An2 Abstract Microfluidics has become an important tool in diagnosing many diseases including neurological and genetic disorders. Alzheimer s disease AD is a neurodegenerative disease that irreversibly and progressively destroys memory language ability and thinking skills. Commonly detection of AD is expensive and complex. Fluorescence in situ hybridization FISH -based microfluidic chip platform is capable of diagnosing AD at an early stage and they are effective tools for the diagnosis with low cost high speed and high sensitivity. In this review we tried to provide basic information on the diagnosis of AD via FISH-based microfluidics. Different sample preparations using a microfluidic chip for diagnosis of AD are highlighted. Moreover rapid innovations in nanotechnology for diagnosis are explained. This review will provide information on dynamic quantification methods for the diagnosis and treatment of AD. The knowledge provided in this review will help develop new integration diagnostic techniques based on FISH and microfluidics. Keywords Fluorescence in situ hybridization FISH Microfluidic chip Alzheimer s disease AD Nanoparticles Molecular probes Introduction Fluorescence in situ hybridization FISH was developed during the 1980s for the detection of specific nucleic acid sequences and cytogenetical analysis 1 2 . Further FISH has replaced conventional methods such as radioisotope probe labeling 3 . Traditional FISH techniques can safely and quantitatively detect many targets but it is a time-consuming process 4 . Recently FISH-based microfluidic technique was introduced and was .
