Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization. | Jenabian et al. AIDS Research and Therapy 2010 7 16 http content 7 1 16 AIDS RESEARCH AND THERAPY RESEARCH Open Access Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization Mohammad-Ali Jenabian 1 Héla Saidi1 Charlotte Charpentier1 Hicham Bouhlal2 Dominique Schols3 Jan Balzarini3 Thomas W Bell4 Guido Vanham5 and Laurent Bélec 1 Abstract Background HIV-1 in genital secretions may be opsonized by several molecules including complement components. Opsonized HIV-1 by complement enhances the infection of various mucosal target cells such as dendritic cells DC and epithelial cells. Results We herein evaluated the effect of HIV-1 complement opsonization on microbicide candidates activity by using three in vitro mucosal models CCR5-tropic HIV-1JR-CSF transcytosis through epithelial cells HIV-1JR-CSF attachment on immature monocyte-derived dendritic cells iMDDC and infectivity of iMDDC by CCR5-tropic HIV-1 BaL and CXCR4-tropic HIV-1 NDK. A panel of 10 microbicide candidates T20 CADA lectines HHA GNA PVAS human lactoferrin and monoclonal antibodies IgG1B12 12G5 2G12 and 2F5 were investigated using cell-free unopsonized or opsonized HIV-1 by complements. Only HHA and PVAS were able to inhibit HIV trancytosis. Upon opsonization transcytosis was affected only by HHA HIV-1 adsorption on iMDDC by four molecules lactoferrin IgG1B12 IgG2G5 IgG2G12 and replication in iMDDC of HIV-1 BaL by five molecules lactoferrin CADA T20 IgG1B12 IgG2F5 and of HIV-1 NDK by two molecules lactoferrin IgG12G5 . Conclusion These observations demonstrate that HIV-1 opsonization by complements may modulate in vitro the efficiency of candidate microbicides to inhibit HIV-1 infection of mucosal target cells as well as its crossing through mucosa. Background Recent disappointing failure in microbicide clinical trials revealed that major gaps in basic and applied knowledges remain to conceive effective microbicide .
