Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Toxic effects of methoxychlor on the episodic prolactin secretory pattern: Possible mediated effects of nitric oxide production. | Journal of Circadian Rhythms BioMed Central Research Open Access Toxic effects of methoxychlor on the episodic prolactin secretory pattern Possible mediated effects of nitric oxide production Anunciación Lafuente1 Teresa Cabaleiro1 Pilar Cano2 and Ana I Esquifino 2 Address 1Laboratorio de Toxicología Facultad de Ciencias Universidad de Vigo Campus de Orense Las Lagunas 32004 Orense Spain and 2Departamento de Bioquímica y Biología Molecular III Facultad de Medicina Universidad Complutense 28040 Madrid Spain Email Anunciación Lafuente - lafuente@ Teresa Cabaleiro - lafuente@ Pilar Cano - pelayos@ Ana I Esquifino - pelayos@ Corresponding author Published 03 March 2006 Received 22 November 2005 Accepted 03 March 2006 Journal of Circadian Rhythms2006 4 3 doi 1740-3391-4-3 This article is available from http content 4 1 3 2006Lafuente et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background This work addresses the issue of whether methoxychlor MTX exposure may modify the ultradian secretion of prolactin through changes in the synthesis of nitric oxide NO induced by Nm-nitro-L-arginine methyl ester L-NAME in the hypothalamic-pituitary axis. Associated changes in dopamine DA content in the anterior AH mediobasal MBH and posterior hypothalamus PH and median eminence ME were evaluated. Methods Two groups of animals MTX and MTX L-NAME treated received subcutaneous sc injections of MTX at a dose of 25 mg kg day for one month. The other two groups of animals control and L-NAME treated received sc vehicle injections mL day of sesame oil during the same period of time to be used as controls. Forty hours before the day of the experiment animals were
