Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Technical phosphoproteomic and bioinformatic tools useful in cancer research. | López et al. Journal of Clinical Bioinformatics 2011 1 26 http content 1 1 26 JOURNAL OF CLINICAL BIOINFORMATICS REVIEW Open Access Technical phosphoproteomic and bioinformatic tools useful in cancer research 1 23 4 2 3 2t Elena Lopez Jan-Jaap Wesselink Isabel López Jesus Mendieta Paulino Gómez-Puertas 1 and Sarbelio Rodríguez Munozs Abstract Reversible protein phosphorylation is one of the most important forms of cellular regulation. Thus phosphoproteomic analysis of protein phosphorylation in cells is a powerful tool to evaluate cell functional status. The importance of protein kinase-regulated signal transduction pathways in human cancer has led to the development of drugs that inhibit protein kinases at the apex or intermediary levels of these pathways. Phosphoproteomic analysis of these signalling pathways will provide important insights for operation and connectivity of these pathways to facilitate identification of the best targets for cancer therapies. Enrichment of phosphorylated proteins or peptides from tissue or bodily fluid samples is required. The application of technologies such as phosphoenrichments mass spectrometry MS coupled to bioinformatics tools is crucial for the identification and quantification of protein phosphorylation sites for advancing in such relevant clinical research. A combination of different phosphopeptide enrichments quantitative techniques and bioinformatic tools is necessary to achieve good phospho-regulation data and good structural analysis of protein studies. The current and most useful proteomics and bioinformatics techniques will be explained with research examples. Our aim in this article is to be helpful for cancer research via detailing proteomics and bioinformatic tools. Introduction Phosphoproteomics plays an important role in our understanding of how phosphorylation participates in translating distinct signals into the normal and or abnormal physiological responses and has shifted .
