Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells. | JOURNAL OF BIOMEDICAL SCIENCE Yang et al. Journal of Biomedical Science 2011 18 59 http content 18 1 59 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan RESEARCH Open Access High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells i- 1 1 1 1 -71 1 3 4 Xu-Fang Yang Xu He Jian He Li-Hong Zhang Xue-Jin Su Zhi-Yong Dong Yun-Jian Xu Yan Li and Yu-Lin Li1 Abstract Background Developing efficient methods to isolate and identify human adipose-derived mesenchymal stem cells hADSCs remains to be one of the major challenges in tissue engineering. Methods We demonstrate here a method by isolating hADSCs from abdominal subcutaneous adipose tissue harvested during caesarian section. The hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks. Results The yield reached around 1 X 106 hADSCs per gram adipose tissue. The following comprehensive identification and characterization illustrated pronounced features of mesenchymal stem cells MSCs . The fibroblast-like hADSCs exhibited typical ultrastructure details for vigorous cell activities. Karyotype mapping showed normal human chromosome. With unique immunophenotypes they were positive for CD29 CD44 CD73 CD105 and CD166 but negative for CD31 CD34 CD45 and HLA-DR. The growth curve and cell cycle analysis revealed high capability for self-renewal and proliferation. Moreover these cells could be functionally induced into adipocytes osteoblasts and endothelial cells in the presence of appropriate conditioned media. Conclusion The data presented here suggest that we have developed high efficient isolation and cultivation methods with a systematic strategy for identification and characterization of hADSCs. These techniques will be able to provide safe and stable seeding cells for research and clinical application. Background Mesenchymal stem cells have been widely used in
