Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart. | Zeng et al. Journal of Biomedical Science 2011 18 67 http content 18 1 67 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Developmental patterns and characteristics of epicardial cell markers Tbx18 and Wt1 in murine embryonic heart Bin Zeng1 Xiao-feng Ren2 Feng Cao1 Xiao-yang Zhou1 and Jing Zhang1 Abstract Background Although recent studies have highlighted the role of epicardial cells during cardiac development and regeneration their cardiomyogenic potential is still controversial due to the question of lineage tracing of epicardial cells. The present study therefore aimed to examine the the expression of Tbx18 and Wt1 in embryonic heart and to identify whether Tbx18 and Wt1 themselves expressed in the cardiomyocyte. Methods Mouse embryonic hearts were collected at different stages for immunofluorescence costaining with either Tbx18 and the cardiac transcription factor or Wilms tumor 1 Wt1 and . Results Tbx18 and Wt1 but not were expressed in the proepicardium and epicardium. Tbx18 was expressed in cells within the heart from to at least these Tbx18-expressing cells were positive except for a few cells that were negative at . Wt1 was expressed in cells within the heart from to at least but these Wt1-expressing cells were negative. Conclusion The data obtained in this study demonstrate that Tbx18 is expressed in the cardiomyocytes from to at least and Wt1 is expressed within the heart from to at least but not in the cardiomyocyte. These findings may provide new insights on the role of the epicardial cells in cardiac regeneration. Keywords Cardiac progenitor Epicardial cells Tbx18 Wt1 Development Background During embryogenesis cells from the proepicardium migrate onto the myocardium to form the epicardium. The proepicardium is a source of .
